Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (36)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Otsuki, T.
Right arrow Articles by Liu, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Otsuki, T.
Right arrow Articles by Liu, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2001, Vol. 10, No. 23 2651-2660
© 2001 Oxford University Press

Fanconi anemia protein, FANCA, associates with BRG1, a component of the human SWI/SNF complex

Tetsuya Otsuki, Yusuke Furukawa1, Keiko Ikeda2, Hitoshi Endo3, Takayuki Yamashita4, Azusa Shinohara5, Akihiko Iwamatsu5, Keiya Ozawa and Johnson M. Liu6,+

Department of Hematology, 1Center for Molecular Medicine, 2Department of Biology and 3Department of Biochemistry, Jichi Medical School, Yakushiji 3311-1, Minamikawachi, kawachi, Tochigi 329-0498, Japan, 4Division of Genetic Diagnosis, Institute of Medical Science, University of Tokyo, Sirokanedai 4-6-1, Minato-ku, Tokyo 108, Japan, 5Section of Protein Chemistry, Central Laboratories for Key Technology, Kirin Brewery Co. Ltd, Fukuura 1-13-5, Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan and 6Hematology Branch, National Heart, Lung and Blood Institute, Building 10, Room 7C103, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA

Fanconi anemia (FA) is a genetic disorder that predisposes to hematopoietic failure, birth defects and cancer. We identified an interaction between the FA protein, FANCA and brm-related gene 1 (BRG1) product. BRG1 is a subunit of the SWI/SNF complex, which remodels chromatin structure through a DNA-dependent ATPase activity. FANCA was demonstrated to associate with the endogenous SWI/SNF complex. We also found a significant increase in the molecular chaperone, glucose-regulated protein 94 (GRP94) among BRG1-associated factors isolated from a FANCA-mutant cell line, which was not seen in either a normal control cell line or the mutant line complemented by wild-type FANCA. Despite this specific difference, FANCA did not appear to be absolutely required for in vitro chromatin remodeling. Finally, we demonstrated co-localization in the nucleus between transfected FANCA and BRG1. The physiological action of FANCA on the SWI/SNF complex remains to be clarified, but our work suggests that FANCA may recruit the SWI/SNF complex to target genes, thereby enabling coupled nuclear functions such as transcription and DNA repair.

+ To whom correspondence should be addressed. Tel: +1 301 496 2452; Fax: +1 301 496 8396; Email: liuj@nhlbi.nih.gov


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
K. W. Trotter, H.-Y. Fan, M. L. Ivey, R. E. Kingston, and T. K. Archer
The HSA Domain of BRG1 Mediates Critical Interactions Required for Glucocorticoid Receptor-Dependent Transcriptional Activation In Vivo
Mol. Cell. Biol., February 15, 2008; 28(4): 1413 - 1426.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
T. Efferth, S. Kahl, K. Paulus, M. Adams, R. Rauh, H. Boechzelt, X. Hao, B. Kaina, and R. Bauer
Phytochemistry and pharmacogenomics of natural products derived from traditional chinese medicine and chinese materia medica with activity against tumor cells
Mol. Cancer Ther., January 1, 2008; 7(1): 152 - 161.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Larder, D. Karali, N. Nelson, and P. Brown
Fanconi Anemia a Is a Nucleocytoplasmic Shuttling Molecule Required for Gonadotropin-Releasing Hormone (GnRH) Transduction of the GnRH Receptor
Endocrinology, December 1, 2006; 147(12): 5676 - 5689.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Zhang, T. Ohta, A. Maruyama, T. Hosoya, K. Nishikawa, J. M. Maher, S. Shibahara, K. Itoh, and M. Yamamoto
BRG1 Interacts with Nrf2 To Selectively Mediate HO-1 Induction in Response to Oxidative Stress
Mol. Cell. Biol., November 1, 2006; 26(21): 7942 - 7952.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. S. Fillingham, J. Garg, N. Tsao, N. Vythilingum, T. Nishikawa, and R. E. Pearlman
Molecular Genetic Analysis of an SNF2/brahma-Related Gene in Tetrahymena thermophila Suggests Roles in Growth and Nuclear Development.
Eukaryot. Cell, August 1, 2006; 5(8): 1347 - 1359.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
P. E. Cohen, S. E. Pollack, and J. W. Pollard
Genetic Analysis of Chromosome Pairing, Recombination, and Cell Cycle Control during First Meiotic Prophase in Mammals
Endocr. Rev., June 1, 2006; 27(4): 398 - 426.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
R. Kinouchi, T. Kinouchi, T. Hamamoto, T. Saito, A. Tavares, T. Tsuru, and S. Yamagami
Distribution of CESP-1 Protein in the Corneal Endothelium and Other Tissues.
Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1397 - 1403.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P. P. Medina, J. Carretero, E. Ballestar, B. Angulo, F. Lopez-Rios, M. Esteller, and M. Sanchez-Cespedes
Transcriptional targets of the chromatin-remodelling factor SMARCA4/BRG1 in lung cancer cells
Hum. Mol. Genet., April 1, 2005; 14(7): 973 - 982.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Mi and G. M. Kupfer
The Fanconi anemia core complex associates with chromatin during S phase
Blood, January 15, 2005; 105(2): 759 - 766.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. Zheng, B. Patel, M. McMenamin, A. M. Paprocki, R. D. Schramm, N. G. Nagl Jr, D. Wilsker, X. Wang, E. Moran, and K. E. Latham
Expression of Genes Encoding Chromatin Regulatory Factors in Developing Rhesus Monkey Oocytes and Preimplantation Stage Embryos: Possible Roles in Genome Activation
Biol Reprod, May 1, 2004; 70(5): 1419 - 1427.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P. Hohenstein and R. Fodde
Of mice and (wo)men: genotype-phenotype correlations in BRCA1
Hum. Mol. Genet., October 15, 2003; 12(90002): R271 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. C.Y. Wong, N. Alon, C. Mckerlie, J. R. Huang, M. S. Meyn, and M. Buchwald
Targeted disruption of exons 1 to 6 of the Fanconi Anemia group A gene leads to growth retardation, strain-specific microphthalmia, meiotic defects and primordial germ cell hypoplasia
Hum. Mol. Genet., August 15, 2003; 12(16): 2063 - 2076.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Yamamoto, M. Ishiai, N. Matsushita, H. Arakawa, J. E. Lamerdin, J.-M. Buerstedde, M. Tanimoto, M. Harada, L. H. Thompson, and M. Takata
Fanconi Anemia FANCG Protein in Mitigating Radiation- and Enzyme-Induced DNA Double-Strand Breaks by Homologous Recombination in Vertebrate Cells
Mol. Cell. Biol., August 1, 2003; 23(15): 5421 - 5430.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. R. Meetei, S. Sechi, M. Wallisch, D. Yang, M. K. Young, H. Joenje, M. E. Hoatlin, and W. Wang
A Multiprotein Nuclear Complex Connects Fanconi Anemia and Bloom Syndrome
Mol. Cell. Biol., May 15, 2003; 23(10): 3417 - 3426.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
M. Bogliolo, O. Cabre, E. Callen, V. Castillo, A. Creus, R. Marcos, and J. Surralles
The Fanconi anaemia genome stability and tumour suppressor network
Mutagenesis, November 1, 2002; 17(6): 529 - 538.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Furukawa, N. Nishimura, Y. Furukawa, M. Satoh, H. Endo, S. Iwase, H. Yamada, M. Matsuda, Y. Kano, and M. Nakamura
Apaf-1 Is a Mediator of E2F-1-induced Apoptosis
J. Biol. Chem., October 11, 2002; 277(42): 39760 - 39768.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P. Pichierri, D. Averbeck, and F. Rosselli
DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein
Hum. Mol. Genet., October 2, 2002; 11(21): 2531 - 2546.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Folias, M. Matkovic, D. Bruun, S. Reid, J. Hejna, M. Grompe, A. D'Andrea, and R. Moses
BRCA1 interacts directly with the Fanconi anemia protein FANCA
Hum. Mol. Genet., October 2, 2002; 11(21): 2591 - 2597.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
E. Callen, M.J. Ramirez, A. Creus, R. Marcos, S. Frias, B. Molina, I. Badell, T. Olive, J.J. Ortega, and J. Surralles
The clastogenic response of the 1q12 heterochromatic region to DNA cross-linking agents is independent of the Fanconi anaemia pathway
Carcinogenesis, August 1, 2002; 23(8): 1267 - 1271.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-S. Dai, N. Chevallier, S. Stone, M. C. Heinrich, M. McConnell, T. Reuter, H. E. Broxmeyer, J. D. Licht, L. Lu, and M. E. Hoatlin
The Effects of the Fanconi Anemia Zinc Finger (FAZF) on Cell Cycle, Apoptosis, and Proliferation Are Differentiation Stage-specific
J. Biol. Chem., July 12, 2002; 277(29): 26327 - 26334.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.