Human Molecular Genetics, 2003, Vol. 12, No. 11 1313-1320
DOI: 10.1093/hmg/ddg137
© 2003 Oxford University Press
Low conservation of alternative splicing patterns in the human and mouse genomes
1Moscow State University, Department of Physics/Biophysics, GSP-2, Leninskie Gory, Moscow 119922, Russia, 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia, 3IntegratedGenomics-Moscow, PO Box 348, Moscow 117333, Russia and 4State Scientific Center GosNIIGenetika, 1st Dorozhny 1, Moscow 113545, Russia
Received January 28, 2003; Revised March 14, 2003; Accepted March 21, 2003
Alternative splicing has recently emerged as a major mechanism of generating protein diversity in higher eukaryotes. We compared alternative splicing isoforms of 166 pairs of orthologous human and mouse genes. As the mRNA and EST libraries of human and mouse are not complete and thus cannot be compared directly, we instead analyzed whether known cassette exons or alternative splicing sites from one genome are conserved in the other genome. We demonstrate that about half of the analyzed genes have species-specific isoforms, and about a quarter of elementary alternatives are not conserved between the human and mouse genomes. The detailed results of this study are available at www.ig-msk.ru:8005/HMG_paper.
* To whom correspondence should be addressed at: State Scientific Center GosNIIGenetika, 1st Dorozhny 1, Moscow 113545, Russia. Tel: +7 0951352041; Fax: +7 0951326080; Email: misha{at}imb.ac.ru
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