Skip Navigation


Human Molecular Genetics Advance Access originally published online on June 30, 2004
Human Molecular Genetics 2004 13(17):1827-1837; doi:10.1093/hmg/ddh195
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
13/17/1827    most recent
ddh195v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (67)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by de Leeuw, R. J.
Right arrow Articles by Lam, W. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Leeuw, R. J.
Right arrow Articles by Lam, W. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, Vol. 13, No. 17 © Oxford University Press 2004; all rights reserved

Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes

Ronald J. de Leeuw1,*, Jonathan J. Davies1, Andreas Rosenwald4,5, Gwyn Bebb2, Randy D. Gascoyne3, Martin J.S. Dyer6, Louis M. Staudt5, Jose A. Martinez-Climent7 and Wan L. Lam1

1Department of Cancer Genetics, 2Department of Medical Oncology, 3Department of Pathology, British Columbia Cancer Agency, Vancouver, BC, Canada, 4Institute of Pathology, University of Wuerzburg, Germany, 5Center for Cancer Research, National Cancer Institute, Bethesda, MA, USA, 6MRC Toxicology Unit, University of Leicester, UK and 7Department of Hematology and Medical Oncology, Hospital Clinico, University of Valencia, Spain

Received May 1, 2004; Accepted June 15, 2004

Mantle cell lymphoma (MCL) is an aggressive non-Hodgkin's lymphoma with median patient survival times of ~3 years. Although the characteristic t(11;14)(q13;q32) is found in virtually all cases, experimental evidence suggests that this event alone is insufficient to result in lymphoma and secondary genomic alterations are required. Using a newly developed DNA microarray of 32 433 overlapping genomic segments spanning the entire human genome, we can for the first time move beyond marker based analysis and comprehensively search for secondary genomic alterations concomitant with the t(11;14) in eight commonly used cell models of MCL (Granta-519, HBL-2, NCEB-1, Rec-1, SP49, UPN-1, Z138C and JVM-2). Examining these genomes at tiling resolution identified an unexpected average of 35 genetic alterations per cell line, with equal numbers of amplifications and deletions. Recurrent high-level amplifications were identified at 18q21 containing BCL2, and at 13q31 containing GPC5. In addition, a recurrent homozygous deletion was identified at 9p21 containing p15 and p16. Alignment of these profiles revealed 14 recurrent losses and 21 recurrent gains as small as 130 kb. Remarkably, even the intra immunoglobulin gene deletions at 2p11 and 22q11 were detected, demonstrating the power of combining the detection sensitivity of array comparative genomic hybridization (CGH) with the resolution of an overlapping whole genome tiling-set. These alterations not only coincided with previously described aberrations in MCL, but also defined 13 novel regions. Further characterization of such minimally altered genomic regions identified using whole genome array CGH will define novel dominant oncogenes and tumor suppressor genes that play important roles in the pathogenesis of MCL.

* To whom correspondence should be addressed at: British Columbia Research Centre, 601 W. 10th Avenue, Vancouver, BC, Canada V5Z 1L3. Tel: +1 6048776000; Fax: +1 6048776155; Email: rdeleeuw{at}bccrc.ca


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
A. Psyrri, S. Papageorgiou, E. Liakata, A. Scorilas, D. Rontogianni, C. K. Kontos, P. Argyriou, D. Pectasides, N. Harhalakis, V. Pappa, et al.
Phosphatidylinositol 3'-Kinase Catalytic Subunit {alpha} Gene Amplification Contributes to the Pathogenesis of Mantle Cell Lymphoma
Clin. Cancer Res., September 15, 2009; 15(18): 5724 - 5732.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
S. P. Shah, K-J. Cheung Jr, N. A. Johnson, G. Alain, R. D. Gascoyne, D. E. Horsman, R. T. Ng, and K. P. Murphy
Model-based clustering of array CGH data
Bioinformatics, June 15, 2009; 25(12): i30 - i38.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K.-J. J. Cheung, S. P. Shah, C. Steidl, N. Johnson, T. Relander, A. Telenius, B. Lai, K. P. Murphy, W. Lam, A. J. Al-Tourah, et al.
Genome-wide profiling of follicular lymphoma by array comparative genomic hybridization reveals prognostically significant DNA copy number imbalances
Blood, January 1, 2009; 113(1): 137 - 148.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. Paoluzzi, M. Gonen, J. R. Gardner, J. Mastrella, D. Yang, J. Holmlund, M. Sorensen, L. Leopold, K. Manova, G. Marcucci, et al.
Targeting Bcl-2 family members with the BH3 mimetic AT-101 markedly enhances the therapeutic effects of chemotherapeutic agents in in vitro and in vivo models of B-cell lymphoma
Blood, June 1, 2008; 111(11): 5350 - 5358.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
B. I. Ferreira, J. F. Garcia, J. Suela, M. Mollejo, F. I. Camacho, A. Carro, S. Montes, M. A. Piris, and J. C. Cigudosa
Comparative genome profiling across subtypes of low-grade B-cell lymphoma identifies type-specific and common aberrations that target genes with a role in B-cell neoplasia
Haematologica, May 1, 2008; 93(5): 670 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
C. A. Tucker, A. I. Kapanen, G. Chikh, B. G. Hoffman, A. H. Kyle, I. M. Wilson, D. Masin, R. D. Gascoyne, M. Bally, and R. J. Klasa
Silencing Bcl-2 in models of mantle cell lymphoma is associated with decreases in cyclin D1, nuclear factor-{kappa}B, p53, bax, and p27 levels
Mol. Cancer Ther., April 1, 2008; 7(4): 749 - 758.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
S. P. Shah, W. L. Lam, R. T. Ng, and K. P. Murphy
Modeling recurrent DNA copy number alterations in array CGH data
Bioinformatics, July 1, 2007; 23(13): i450 - i458.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
T. Ripperger, N. von Neuhoff, K. Kamphues, M. Emura, U. Lehmann, M. Tauscher, M. Schraders, P. Groenen, B. Skawran, C. Rudolph, et al.
Promoter methylation of PARG1, a novel candidate tumor suppressor gene in mantle cell lymphomas
Haematologica, April 1, 2007; 92(4): 460 - 468.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
I. Wlodarska, C. Matthews, E. Veyt, H. Pospisilova, M. A. Catherwood, T. S. Poulsen, V. Vanhentenrijk, R. Ibbotson, P. Vandenberghe, T.C.M. C. Morris, et al.
Telomeric IGH Losses Detectable by Fluorescence in Situ Hybridization in Chronic Lymphocytic Leukemia Reflect Somatic VH Recombination Events
J. Mol. Diagn., February 1, 2007; 9(1): 47 - 54.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. Mestre-Escorihuela, F. Rubio-Moscardo, J. A. Richter, R. Siebert, J. Climent, V. Fresquet, E. Beltran, X. Agirre, I. Marugan, M. Marin, et al.
Homozygous deletions localize novel tumor suppressor genes in B-cell lymphomas
Blood, January 1, 2007; 109(1): 271 - 280.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
T Ohguri, M Hisaoka, S Kawauchi, K Sasaki, T Aoki, S Kanemitsu, A Matsuyama, Y Korogi, and H Hashimoto
Cytogenetic analysis of myxoid liposarcoma and myxofibrosarcoma by array-based comparative genomic hybridisation
J. Clin. Pathol., September 1, 2006; 59(9): 978 - 983.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
P. L. Rosa, E. Viara, P. Hupe, G. Pierron, S. Liva, P. Neuvial, I. Brito, S. Lair, N. Servant, N. Robine, et al.
VAMP: Visualization and analysis of array-CGH, transcriptome and other molecular profiles
Bioinformatics, September 1, 2006; 22(17): 2066 - 2073.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Rudelius, S. Pittaluga, S. Nishizuka, T. H.-T. Pham, F. Fend, E. S. Jaffe, L. Quintanilla-Martinez, and M. Raffeld
Constitutive activation of Akt contributes to the pathogenesis and survival of mantle cell lymphoma
Blood, September 1, 2006; 108(5): 1668 - 1676.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
C. Rouveirol, N. Stransky, Ph. Hupe, Ph. L. Rosa, E. Viara, E. Barillot, and F. Radvanyi
Computation of recurrent minimal genomic alterations from array-CGH data
Bioinformatics, April 1, 2006; 22(7): 849 - 856.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. V. Beum, A. D. Kennedy, M. E. Williams, M. A. Lindorfer, and R. P. Taylor
The Shaving Reaction: Rituximab/CD20 Complexes Are Removed from Mantle Cell Lymphoma and Chronic Lymphocytic Leukemia Cells by THP-1 Monocytes
J. Immunol., February 15, 2006; 176(4): 2600 - 2609.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T.-M. Kim, S.-H. Yim, J.-S. Lee, M.-S. Kwon, J.-W. Ryu, H.-M. Kang, H. Fiegler, N. P. Carter, and Y.-J. Chung
Genome-Wide Screening of Genomic Alterations and Their Clinicopathologic Implications in Non-Small Cell Lung Cancers
Clin. Cancer Res., December 1, 2005; 11(23): 8235 - 8242.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. Rubio-Moscardo, D. Blesa, C. Mestre, R. Siebert, T. Balasas, A. Benito, A. Rosenwald, J. Climent, J. I. Martinez, M. Schilhabel, et al.
Characterization of 8p21.3 chromosomal deletions in B-cell lymphoma: TRAIL-R1 and TRAIL-R2 as candidate dosage-dependent tumor suppressor genes
Blood, November 1, 2005; 106(9): 3214 - 3222.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. Rubio-Moscardo, J. Climent, R. Siebert, M. A. Piris, J. I. Martin-Subero, I. Nielander, J. Garcia-Conde, M. J. S. Dyer, M. J. Terol, D. Pinkel, et al.
Mantle-cell lymphoma genotypes identified with CGH to BAC microarrays define a leukemic subgroup of disease and predict patient outcome
Blood, June 1, 2005; 105(11): 4445 - 4454.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. G. Buckley, C. Jarbo, U. Menzel, T. Mathiesen, C. Scott, S. G. Gregory, C. F. Langford, and J. P. Dumanski
Comprehensive DNA Copy Number Profiling of Meningioma Using a Chromosome 1 Tiling Path Microarray Identifies Novel Candidate Tumor Suppressor Loci
Cancer Res., April 1, 2005; 65(7): 2653 - 2661.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Schraders, R. Pfundt, H. M. P. Straatman, I. M. Janssen, A. G. van Kessel, E. F. P. M. Schoenmakers, J. H. J. M. van Krieken, and P. J. T. A. Groenen
Novel chromosomal imbalances in mantle cell lymphoma detected by genome-wide array-based comparative genomic hybridization
Blood, February 15, 2005; 105(4): 1686 - 1693.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.