Human Molecular Genetics Advance Access originally published online on September 10, 2008
Human Molecular Genetics 2008 17(24):3942-3952; doi:10.1093/hmg/ddn296
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CHIP deletion reveals functional redundancy of E3 ligases in promoting degradation of both signaling proteins and expanded glutamine proteins
1 Department of Pharmacology 2 Department of Pathology, The University of Michigan Medical School, 3510 MSRB I, 1150 W. Medical Center Dr., Ann Arbor, MI 48109, USA
* To whom correspondence should be addressed. Tel: +1 7346474624; Fax: +1 7346153441; Email: liebermn{at}umich.edu
Received July 8, 2008; Revised August 22, 2008; Accepted September 9, 2008
CHIP (carboxy terminus of Hsc70-interacting protein) an E3 ubiquitin ligase that binds to Hsp70 and Hsp90, promotes degradation of several Hsp90-regulated signaling proteins and disease-causing proteins containing expanded glutamine tracts. In polyglutamine disease models, CHIP has been considered a primary protection factor by promoting degradation of these misfolded proteins. Here, we show that two CHIP substrates, the glucocorticoid receptor (GR), a classic Hsp90-regulated signaling protein, and the expanded glutamine androgen receptor (AR112Q), are degraded at the same rate in CHIP–/– and CHIP+/+ mouse embryonic fibroblasts after treatment with the Hsp90 inhibitor geldanamycin. CHIP–/– cytosol has the same ability as CHIP+/+ cytosol to ubiquitinate purified neuronal nitric oxide synthase (nNOS), another established CHIP substrate. To determine whether other E3 ubiquitin ligases that bind to Hsp70 (Parkin) or Hsp90 (Mdm2) act on CHIP substrates, each E3 ligase was co-expressed with the GR, nNOS, AR112Q or Q78 ataxin-3. CHIP lowered the levels of all four proteins, Parkin acted on nNOS and Q78 ataxin-3 but not on the steroid receptors, and Mdm2 did not affect any of the co-expressed proteins. Moreover, both CHIP and Parkin co-localized to aggregates of the expanded glutamine AR formed in cell culture and in a knock-in mouse model of spinal and bulbar muscular atrophy. These observations establish that CHIP does not play an exclusive role in regulating the turnover of Hsp90 client signaling proteins or expanded glutamine tract proteins, and show that the Hsp70-dependent E3 ligase Parkin acts redundantly to CHIP on some substrates.
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