© 1993 Oxford University Press
RESEARCH-ARTICLE |
A linkage map of human chromosome 15 with an average resolution of 2 cM and containing 55 polymorphic microsatellites
Centre d'Etude du Polymorphtsme Human 27 rue Juliette Dodu, 75010, Paris, France 1Department of Pediatncs, University of Texas Southwestern Medical Center 6000 Harry Hines Boulevard, Dallas, TX 75235–8591, USA 2Centre d'Etude du Polymorphtsme Human, Genethon, 1 Rue de I'Internatinate 91000 Evry, France
+These authors contributed equally to this work
Received June 23, 1993; Revised September 22, 1993; Accepted October 11, 1993
We have constructed a 2.0 centiMorgan (cM) resolution genetic linkage map for chromosome 15q that contains 55 polymorphic satellites and 3 RFLPs that have placed on the map with odds for order of at least 1,000: 1. Genotypes from 67 polymorphic loci (64 polymorphic microsatellites) were used to construct the map. Nine genes are Included in the 1,000: 1 map and 37 markers have heterozygoslties of at least 70%. The sex-equal map length is 128 cM and the largest genetic interval is 11 cM (15.5 cM on the female map). The female and male map lengths are 150 cM and 106 cM, respectively. The map was constructed with ‘MultiMap’ and is based on the CEPH reference pedigrees and includes over 12, 000 new genotypes. A sub-set of 12 markers spanning the length of the linkage map were genotyped in a somatic cell hybrid panel with breakpoints that divided 15q into five segments. Cytogenetic placement agreed with the linkage positions for each of the microsatellites tested with the exception of one (ACTC) which failed to give consistent results. Ten spontaneous new mutations were identified from a subset of 42 polymorphic microsatellites (out of a total of 20, 420 transmissions), giving an apparent observed spontaneous mutation rate of 5 x 10–4 per locus. An integrated map of chromosome 15q was also constructed with the microsatellite markers described here and previously genotyped RFLP-based markers. The sex average map spans 144.7 cM with an average distance between unique map locations of 3.5 cM and a maximum intermarker distance of 11.5 cM. These genetic linkage maps can be considered baseline maps for 15q which will be useful for physical mapping and the localization of disease genes and other genes of interest.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Katsumata, M. Ohtake, T. Hojo, E. Ogawa, T. Hara, N. Sato, and T. Tanaka Compound Heterozygous Mutations in the Cholesterol Side-Chain Cleavage Enzyme Gene (CYP11A) Cause Congenital Adrenal Insufficiency in Humans J. Clin. Endocrinol. Metab., August 1, 2002; 87(8): 3808 - 3813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. D. Bushby Making sense of the limb-girdle muscular dystrophies Brain, August 1, 1999; 122(8): 1403 - 1420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Christian, N. K. Bhatt, S. A. Martin, J. S. Sutcliffe, T. Kubota, B. Huang, A. Mutirangura, A. C. Chinault, A. L. Beaudet, and D. H. Ledbetter Integrated YAC Contig Map of the Prader-Willi/Angelman Region on Chromosome 15q11-q13 with Average STS Spacing of 35 kb Genome Res., February 1, 1998; 8(2): 146 - 157. [Abstract] [Full Text]
|
|
|
|
 |
|
 J Tomfohrde, A Silverman, R Barnes, M. Fernandez-Vina, M Young, D Lory, L Morris, K. Wuepper, P Stastny, A Menter, et al. Gene for familial psoriasis susceptibility mapped to the distal end of human chromosome 17q Science, May 20, 1994; 264(5162): 1141 - 1145. [Abstract] [PDF]
|
|