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© 1994 Oxford University Press

RESEARCH-ARTICLE

A YAC clone map spanning 7.5 megabases of human chromosome band Xq28

Ute C. Rogner, Petra Kioschis, Klaus Wilke, Wellong Gong+, Elke Pick, Alexander Dietrich§, Ulrich Zechner1, Horst Hameister1, Antonella Pragllola2, Gail E. Herman2, John R.W. Yates3,5, Hans Lehrach4 and Annemarie Poustka*

Deutsches Krebsforschungszentrum Im Neuenheimer Feld 280, 69120 Heidelberg 1Department of Clinical Genetics, University of Ulm Albert-Einstein-Allee 11, 89081 Ulm, Germany 2Department of Molecular and Human Genetics One Baylor Plaza 821T, Houston, TX 77030, USA 3Department of Pathology, University of Cambridge Cambridge CB2 1QP 4lmpenal Cancer Research Fund PO Box 123, 44 Lincoln's Inn Fields, London WC2A 3PX 5Department of Clinical Genetics, Addenbrooke's Hospital Box 134, Cambridge CB2 2QQ, UK

*To whom correspondence should be addressed

Received July 1, 1994; Revised October 6, 1994; Accepted October 6, 1994

Xq28 has been of special Interest in human genetics because a large number of diseases map to this region. As a step In the molecular analysis of the as yet uncloned disease genes, and as a test for the detailed analysis of larger regions of the genome, we have constructed YAC clone contlgs covering the 7.5 Mb region between IDS to the telomere on the long arm of the human X chromosome. Contlgs were assembled and verified by an Integrated hybridization-based strategy. Data was combined from the physical map, from YAC and cosmld mapping experiments, and from the localization of specific transcripts In the region. Two gaps in the YAC map of 250 and 100 kb were covered In part by the aid of cosmid clones, but small gaps of 50 kb each remain. The cloned region is expected to contain yet unidentified genes for at least ten genetic diseases. The construction of ordered YAC clone contlgs of Xq28 represents an Important step In the molecular Identification of these genes, and the further analysis of one of the genetically most Interesting regions of the human genome.

Present addresses: +The Children's Hospital of Philadelphia, Division of Human Genetics and Molecular Biology, 34th Street, Philadelphia, PA 19104, USA

§Department of Pharmacology and Toxicology, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany


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