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© 1994 Oxford University Press

OTHER

A novel nuclear protein binds centromeric alpha satellite DNA

C. Gaff, D. du Sart, P. KalltsIs, R. lannello, A. Nagy and K.H.A. Choo*

Murdoch Institute for Research into Birth Defects, Royal Children's Hospital Remington Road, Parkville, Victoria 3052, Australia

* To whom correspondence should be addressed

Received February 16, 1994; Accepted March 3, 1994

We have previously reported the identification of a naturally occurring junction between alpha satellite and satellite III DNA on human chromosomes 13, 14 and 21 (1). Direct sequence analysis has shown that the 9 bp alphoid-derived direct repeat sequence (GTGAAAAA-G) present at the junction Is fully conserved on these chromosomes. A novel protein, pJ{alpha}, present in HeLa nuclear extracts, binds to the conserved junction sequence. Mutation analysis of the binding site suggests that pJ{alpha} can recognize one of the two 9 bp repeats and provides some Insight into nucleotides that are important for binding. Competition studies support the possibility that this protein binds a significant portion of genomlc alpha satellite DNA. Preliminary protein purification experiments have shown that pJ{alpha} has a molecular weight of 10–15 kDa.


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