© 1994 Oxford University Press
RESEARCH-ARTICLE |
Dissecting the centromere of the human Y chromosome with cloned telomeric DNA
CRC Chromosome Molecular Biology Group, Biochemistry Department, Oxford University South Parks Road, Oxford 0X1 3QU 1John Innes Institute, Colney Lane Norwich, Norfolk NR4 7UH 2Institute of Molecular Medicine, John Radcliffe Hospital Headington, Oxford OX3 9DU, UK
*To whom correspondence should be addressed
Received June 9, 1994; Accepted June 17, 1994
We have used telomeric DNA to break the human Y chromosome within the centromeric array of alphold satellite DNA and have created two derivative chromosomes; one consists of the short arm and 140 kb of alphoid DNA, the other consists of the long arm and 480 kb of alphoid DNA. Both segregate accurately at mitosis. It Is known that there is no large scale sequence duplication around the alphoid DNA and so the simplest Interpretation of our results is that the sequence responsible for accurate segregation is the alphoid DNA Itself. Although the long arm acrocentrlc derivative segregates accurately it lags with respect to the other chromosomes In about 10% of anaphase cells and thus additional sequences may be required for orderly segregation. The short arm acrocentric chromosome Is probably no larger than 12 Mb In size and thus our results also demonstrate that chromosomes of this size are capable of accurate segregation.
+Present address: ICRF Laboratories, PO Box 123, Lincoln‘s Inn Fields, London WC2A 3PX, UK
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Xu, N. C. O. Lee, F. Dafhnis-Calas, S. Malla, M. C. M. Smith, and W. R. A. Brown Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage {phi}BT1 Nucleic Acids Res., January 17, 2008; 36(1): e9 - e9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kouprina, T. Ebersole, M. Koriabine, E. Pak, I. B. Rogozin, M. Katoh, M. Oshimura, K. Ogi, M. Peredelchuk, G. Solomon, et al. Cloning of human centromeres by transformation-associated recombination in yeast and generation of functional human artificial chromosomes Nucleic Acids Res., February 1, 2003; 31(3): 922 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Voet, J. Vermeesch, A. Carens, J. Dürr, C. Labaere, H. Duhamel, G. David, and P. Marynen Efficient Male and Female Germline Transmission of a Human Chromosomal Vector in Mice Genome Res., January 1, 2001; 11(1): 124 - 136. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Okabe, A. Eguchi, A. Masago, T. Hayakawa, and M. Nakanishi TRF1 is a critical trans-acting factor required for de novo telomere formation in human cells Hum. Mol. Genet., November 1, 2000; 9(18): 2639 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Orbach, L. Farrall, J. A. Sweigard, F. G. Chumley, and B. Valent A Telomeric Avirulence Gene Determines Efficacy for the Rice Blast Resistance Gene Pi-ta PLANT CELL, November 1, 2000; 12(11): 2019 - 2032. [Abstract] [Full Text]
|
|
|
|
|
|
T. A. Ebersole, A. Ross, E. Clark, N. McGill, D. Schindelhauer, H. Cooke, and B. Grimes Mammalian artificial chromosome formation from circular alphoid input DNA does not require telomere repeats Hum. Mol. Genet., July 1, 2000; 9(11): 1623 - 1631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Partridge, B. Borgstrøm, and R. C. Allshire Distinct protein interaction domains and protein spreading in a complex centromere Genes & Dev., April 1, 2000; 14(7): 783 - 791. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Henikoff, K. Ahmad, J. S. Platero, and B. van Steensel From the Cover: Heterochromatic deposition of centromeric histone H3-like proteins PNAS, January 18, 2000; 97(2): 716 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ekwall, G. Cranston, and R. C. Allshire Fission Yeast Mutants That Alleviate Transcriptional Silencing in Centromeric Flanking Repeats and Disrupt Chromosome Segregation Genetics, November 1, 1999; 153(3): 1153 - 1169. [Abstract] [Full Text]
|
|
|
|
|
|
A. W.I. Lo, G. C.-C. Liao, M. Rocchi, and K.H. A. Choo Extreme Reduction of Chromosome-Specific alpha -Satellite Array Is Unusually Common in Human Chromosome 21 Genome Res., October 1, 1999; 9(10): 895 - 908. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Henning, E. A. Novotny, S. T. Compton, X.-Y. Guan, P. P. Liu, and M. A. Ashlock Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences PNAS, January 19, 1999; 96(2): 592 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kouprina, K. Kawamoto, J. C. Barrett, V. Larionov, and M. Koi Rescue of Targeted Regions of Mammalian Chromosomes by in Vivo Recombination in Yeast Genome Res., June 1, 1998; 8(6): 666 - 672. [Abstract] [Full Text]
|
|
|
|
|
|
E. K. Round, S. K. Flowers, and E. J. Richards Arabidopsis thaliana Centromere Regions: Genetic Map Positions and Repetitive DNA Structure Genome Res., November 1, 1997; 7(11): 1045 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Halverson, M. Baum, J. Stryker, J. Carbon, and L. Clarke A Centromere DNA-binding Protein from Fission Yeast Affects Chromosome Segregation and Has Homology to Human CENP-B J. Cell Biol., February 10, 1997; 136(3): 487 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Pluta, A. M. Mackay, A. M. Ainsztein, I. G. Goldberg, and W. C. Earnshaw The Centromere: Hub of Chromosomal Activities Science, December 8, 1995; 270(5242): 1591 - 1594. [Abstract] [PDF]
|
|