© 1995 Oxford University Press
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Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues
1Department of Physiology, Johns Hopkins University School of Medicine Baltimore, MD 21287, USA 2Center for Medical Genetics, Johns Hopkins University School of Medicine Baltimore, MD 21287, USA 3Department of Pediatrics, Johns Hopkins University School of Medicine Baltimore, MD 21287, USA 4Department of Molecular Biology and Genetics, Wayne State University School of Medicine Detroit, MI 48201, USA
*To whom correspondence should be addressed
Received September 15, 1994; Revised December 22, 1994; Accepted December 22, 1994
We have cloned a cDNA from the human epithelial cell line T84 whose predicted amino acid sequence shows 93.9% identity with rat CIC-2. Mapping by somatic cell hybrids and polymerase chain reaction localizes the gene corresponding to this cDNA to chromosome 3q26-qter. The major transcription start site assessed by RNA primer extension is 100 nt upstream of the putative translation initiation codon. Analysis of the 5' flanking sequence revealed a high GC content and lack of common transcriptional elements such as TATA and CCAAT boxes. Northern blot analysis indicated wide organ distribution including tissues affected in cystic fibrosis (CF) and expression in an airway epithelial cell line derived from a CF patient. The high degree of sequence similarity and similar tissue distribution to rat CIC-2 suggests that this cDNA encodes the human CIC-2 voltage—gated chloride channel. Since this chloride channel is present in epithelial tissues it may be amenable to manipulation to circumvent the chloride secretion defect observed in CF.
+Current address: National Institute on Aging, Gerontology Research Center, Baltimore, MD 21224, USA
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