Human Molecular Genetics, Vol 5, 339-346, Copyright © 1996 by Oxford University Press
G Gyapay, K Schmitt, C Fizames, H Jones, N Vega-Czarny, D Spillett, D Muselet, JF Prud'Homme, C Dib, C Auffray, J Morissette, J Weissenbach and PN Goodfellow
We have developed a panel of whole-genome radiation hybrids by fusing
irradiated diploid human fibroblasts with recipient hamster cells. This
panel of 168 cell lines has been typed with microsatellite markers of known
genetic location. Of 711 AFM genetic markers 404 were selected to construct
a robust framework map that spans all the autosomes and the X chromosome.
To demonstrate the utility of the panel, 374 expressed sequence tags (ESTs)
previously assigned to chromosomes 1, 2, 14 and 16 were localized on this
map. All of these ESTs could be positioned by pairwise linkage to one of
the framework markers with a LOD score of greater than 8. The whole genome
radiation hybrid panel described here has been used as the starting
material for the Genebridge4 panel that is being made widely available for
genome mapping projects.
ARTICLES
A radiation hybrid map of the human genome
CNRS URA 1922, Genethon, Evry, France.
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I. H. Still, P. Vince, and J. K. Cowell Direct isolation of human transcribed sequences from yeast artificial chromosomes through the application of RNA fingerprinting PNAS, September 16, 1997; 94(19): 10373 - 10378. [Abstract] [Full Text] [PDF]
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M. Nagira, T. Imai, K. Hieshima, J. Kusuda, M. Ridanpaa, S. Takagi, M. Nishimura, M. Kakizaki, H. Nomiyama, and O. Yoshie Molecular Cloning of a Novel Human CC Chemokine Secondary Lymphoid-Tissue Chemokine That Is a Potent Chemoattractant for Lymphocytes and Mapped to Chromosome 9p13 J. Biol. Chem., August 1, 1997; 272(31): 19518 - 19524. [Abstract] [Full Text] [PDF]
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F. Bulle, N. Chiannilkulchai, A. Pawlak, J. Weissenbach, G. Gyapay, and G. Guellaen Identification and Chromosomal Localization of Human Genes Containing CAG/CTG Repeats Expressed in Testis and Brain Genome Res., July 1, 1997; 7(7): 705 - 715. [Abstract] [Full Text] [PDF]
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D. Botstein and J. M. Cherry Molecular linguistics: Extracting information from gene and protein sequences PNAS, May 27, 1997; 94(11): 5506 - 5507. [Full Text] [PDF]
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A. Collins, J. Frezal, J. Teague, and N. E. Morton A metric map of humans: 23,500 loci in 850 bands PNAS, December 10, 1996; 93(25): 14771 - 14775. [Abstract] [Full Text] [PDF]
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