Human Molecular Genetics, Vol 5, 989-993, Copyright © 1996 by Oxford University Press
T Takahara, T Ohsumi, J Kuromitsu, K Shibata, N Sasaki, Y Okazaki, H Shibata, S Sato, A Yoshiki, M Kusakabe, M Muramatsu, M Ueki, K Okuda and Y Hayashizaki
We examined the genomic structure of the reeler gene in Orleans reeler
mouse mutant. Exon skipping of the reeler gene caused a 220 bp deletion in
the transcript, resulting in a frame shift of the reeler gene which
disrupts the 8th EGF-like motif of the reeler product. Surprisingly, the
skipped exon was inserted by the 7104 bp L1 element which carried the
full-length stretch of the mouse L1 sequence, consisting of a 212 bp F-type
tandem repeat, open reading frame 1 (ORF1), ORF2, the polyadenylation
signal and a poly A stretch. The transposed L1 sequence was flanked by 13
bp of the target sequence at both ends. ORF1 and ORF2 of this L1 repeat
element are thought to encode a component of the RNP particle and the
reverse transcriptase, respectively. Orleans reeler was originally
established by spontaneous mutation caused by L1 insertion, and this L1
sequence is considered to be potentially active for transposition in mouse
genome.
ARTICLES
Dysfunction of the Orleans reeler gene arising from exon skipping due to transposition of a full-length copy of an active L1 sequence into the skipped exon
Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Ibaraki, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Teneng, V. Stribinskis, and K. S. Ramos Context-specific regulation of LINE-1 Genes Cells, October 1, 2007; 12(10): 1101 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Andrade, V. Komnenovic, S. M. Blake, Y. Jossin, B. Howell, A. Goffinet, W. J. Schneider, and J. Nimpf ApoER2/VLDL receptor and Dab1 in the rostral migratory stream function in postnatal neuronal migration independently of Reelin PNAS, May 15, 2007; 104(20): 8508 - 8513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, A. Rattner, and J. Nathans Effects of L1 retrotransposon insertion on transcript processing, localization and accumulation: lessons from the retinal degeneration 7 mouse and implications for the genomic ecology of L1 elements Hum. Mol. Genet., July 1, 2006; 15(13): 2146 - 2156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Hammond, E. So, J. Gunnersen, H. Valcanis, M. Kalloniatis, and S.-S. Tan Layer Positioning of Late-Born Cortical Interneurons Is Dependent on Reelin But Not p35 Signaling J. Neurosci., February 1, 2006; 26(5): 1646 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rossel, K. Loulier, C. Feuillet, S. Alonso, and P. Carroll Reelin signaling is necessary for a specific step in the migration of hindbrain efferent neurons Development, March 15, 2005; 132(6): 1175 - 1185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. Lu and K. S. Ramos Redox Regulation of a Novel L1Md-A2 Retrotransposon in Vascular Smooth Muscle Cells J. Biol. Chem., July 18, 2003; 278(30): 28201 - 28209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shen, E. M. Sturgis, S. G. Khan, Y. Qiao, T. Shahlavi, S. A. Eicher, Y. Xu, X. Wang, S. S. Strom, M. R. Spitz, et al. An Intronic Poly (AT) Polymorphism of the DNA Repair Gene XPC and Risk of Squamous Cell Carcinoma of the Head and Neck: A Case-Control Study Cancer Res., April 1, 2001; 61(8): 3321 - 3325. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. G. Khan, E.J. Metter, R. E. Tarone, V. A. Bohr, L. Grossman, M. Hedayati, S. J. Bale, S. Emmert, and K. H. Kraemer A new xeroderma pigmentosum group C poly(AT) insertion/deletion polymorphism Carcinogenesis, October 1, 2000; 21(10): 1821 - 1825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aguilo, T. H. Schwartz, V. S. Kumar, Z. A. Peterlin, A. Tsiola, E. Soriano, and R. Yuste Involvement of Cajal-Retzius Neurons in Spontaneous Correlated Activity of Embryonic and Postnatal Layer 1 from Wild-Type and Reeler Mice J. Neurosci., December 15, 1999; 19(24): 10856 - 10868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gitton, M. Cohen-Tannoudji, and M. Wassef Role of Thalamic Axons in the Expression of H-2Z1, a Mouse Somatosensory Cortex Specific Marker Cereb Cortex, September 1, 1999; 9(6): 611 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U DeSilva, G D'Arcangelo, V V Braden, J Chen, G G Miao, T Curran, and E D Green The human reelin gene: isolation, sequencing, and mapping on chromosome 7. Genome Res., February 1, 1997; 7(2): 157 - 164. [Abstract] [PDF]
|
|
|
|
|
|
G. D'Arcangelo, K. Nakajima, T. Miyata, M. Ogawa, K. Mikoshiba, and T. Curran Reelin Is a Secreted Glycoprotein Recognized by the CR-50 Monoclonal Antibody J. Neurosci., January 1, 1997; 17(1): 23 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Goodier, E. M. Ostertag, K. Du, and H. H. Kazazian Jr. A Novel Active L1 Retrotransposon Subfamily in the Mouse Genome Res., October 1, 2001; 11(10): 1677 - 1685. [Abstract] [Full Text] [PDF]
|
|