Human Molecular Genetics, Vol 6, 451-456, Copyright © 1997 by Oxford University Press
GJ Brock and A Bird
The pattern of methylation in human genes for 18S and 28S ribosomal RNA has
been investigated using methylation-sensitive restriction enzymes. We find
that the transcribed region of the repeat unit is predominantly
unmethylated, in agreement with previous studies. In contrast the non-
transcribed spacer, which makes up the majority of the 43 kb repeat unit,
is highly methylated in blood cell DNA. The boundaries between methylated
and non-methylated domains appear to be relatively sharp, and occur
approximately 1.5 kb upstream of the 5' edge of the proximal promoter and
approximately 1.0 kb downstream of the 3' end of the transcribed region. A
small proportion of all repeat units are methylated throughout the
transcribed region, and may represent silent genes. The coincidence between
the methylation pattern, the transcription pattern and other features of
the repeat unit has implications for our understanding of the mechanism by
which patterns of DNA methylation are generated.
ARTICLES
Mosaic methylation of the repeat unit of the human ribosomal RNA genes
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Koukalova, M. Fojtova, K. Y. Lim, J. Fulnecek, A. R. Leitch, and A. Kovarik Dedifferentiation of Tobacco Cells Is Associated with Ribosomal RNA Gene Hypomethylation, Increased Transcription, and Chromatin Alterations Plant Physiology, September 1, 2005; 139(1): 275 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Espada, E. Ballestar, M. F. Fraga, A. Villar-Garea, A. Juarranz, J. C. Stockert, K. D. Robertson, F. Fuks, and M. Esteller Human DNA Methyltransferase 1 Is Required for Maintenance of the Histone H3 Modification Pattern J. Biol. Chem., August 27, 2004; 279(35): 37175 - 37184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Oakes, D. J. Smiraglia, C. Plass, J. M. Trasler, and B. Robaire Aging results in hypermethylation of ribosomal DNA in sperm and liver of male rats PNAS, February 18, 2003; 100(4): 1775 - 1780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. R. Brock, T. H.-M. Huang, C.-M. Chen, and K. J. Johnson A novel technique for the identification of CpG islands exhibiting altered methylation patterns (ICEAMP) Nucleic Acids Res., December 15, 2001; 29(24): e123 - e123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Yan, M. R. Perry, D. E. Laux, A. L. Asare, C. W. Caldwell, and T. H.-M. Huang CpG Island Arrays: An Application toward Deciphering Epigenetic Signatures of Breast Cancer Clin. Cancer Res., April 1, 2000; 6(4): 1432 - 1438. [Abstract] [Full Text]
|
|
|
|
 |
|
 H.-H. Ng, P. Jeppesen, and A. Bird Active Repression of Methylated Genes by the Chromosomal Protein MBD1 Mol. Cell. Biol., February 15, 2000; 20(4): 1394 - 1406. [Abstract] [Full Text]
|
|
|
|
 |
|
 A Akhmanova, T Verkerk, A Langeveld, F Grosveld, and N Galjart Characterisation of transcriptionally active and inactive chromatin domains in neurons J. Cell Sci., January 12, 2000; 113(24): 4463 - 4474. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Héliot, F. Mongelard, C. Klein, M.-F. O'Donohue, J.-M. Chassery, M. Robert-Nicoud, and Y. Usson Nonrandom Distribution of Metaphase AgNOR Staining Patterns on Human Acrocentric Chromosomes J. Histochem. Cytochem., January 1, 2000; 48(1): 13 - 20. [Abstract] [Full Text]
|
|
|
|
|
|
T. L. McDowell, R. J. Gibbons, H. Sutherland, D. M. O'Rourke, W. A. Bickmore, A. Pombo, H. Turley, K. Gatter, D. J. Picketts, V. J. Buckle, et al. Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes PNAS, November 23, 1999; 96(24): 13983 - 13988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shiraishi, Y. H. Chuu, and T. Sekiya Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis PNAS, March 16, 1999; 96(6): 2913 - 2918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Payen, T. Verkerk, D. Michalovich, S. D. Dreyer, A. Winterpacht, B. Lee, C. I. De Zeeuw, F. Grosveld, and N. Galjart The Centromeric/Nucleolar Chromatin Protein ZFP-37 May Function to Specify Neuronal Nuclear Domains J. Biol. Chem., April 10, 1998; 273(15): 9099 - 9109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Zurita, R Jimenez, M Burgos, and R. de la Guardia Sequential silver staining and in situ hybridization reveal a direct association between rDNA levels and the expression of homologous nucleolar organizing regions: a hypothesis for NOR structure and function J. Cell Sci., January 5, 1998; 111(10): 1433 - 1439. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Z. Strichman-Almashanu, R. S. Lee, P. O. Onyango, E. Perlman, F. Flam, M. B. Frieman, and A. P. Feinberg A Genome-Wide Screen for Normally Methylated Human CpG Islands That Can Identify Novel Imprinted Genes Genome Res., April 1, 2002; 12(4): 543 - 554. [Abstract] [Full Text] [PDF]
|
|