Human Molecular Genetics, 1999, Vol. 8, No. 9 1673-1681
© 1999 Oxford University Press
Isolation, characterization and mutation analysis of PEX13-defective Chinese hamster ovary cell mutants
1Department of Biology, Kyushu University Graduate School of Science, Fukuoka 812-8581, Japan, 2CREST, Japan Science and Technology Corporation, Tokyo, 170-0013, Japan, 3Department of Pediatrics, Gifu University Faculty of Medicine, Gifu 500-8076, Japan and 4Department of Clinical Biochemistry and Pediatrics, Academic Medical Centre, University of Amsterdam, PO Box 22700, 1100DE, Amsterdam, The Netherlands
We isolated peroxisome biogenesis mutants ZP128 and ZP150 from rat PEX2-transformed Chinese hamster ovary (CHO) cells, by the 9-(1"-pyrene)nonanol/ultraviolet method. The mutants lacked morphologically recognizable peroxisomes and showed a typical peroxisome assembly-defective phenotype such as a high sensitivity to 12-(1"-pyrene)dodecanoic acid/UV treatment. By means of PEX cDNA transfection and cell fusion, ZP128 and ZP150 were found to belong to a recently identified complementation group H. Expression of human PEX13 cDNA restored peroxisome assembly in ZP128 and ZP150. CHO cell PEX13 was isolated; its deduced sequence comprises 405 amino acids with 93% identity to human Pex13p. Mutation in PEX13 of mutant ZP150 was determined by RT–PCR: G to A transition resulted in one amino acid substitution, Ser319Asn, in one allele and truncation of a 42 amino acid sequence from Asp265 to Lys306 in another allele. Therefore, ZP128 and ZP150 are CHO cell lines with a phenotype of impaired PEX13.
+ To whom correspondence should be addressed at: Department of Biology, Kyushu University Graduate School of Science, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Tel: +81 92 642 2635; Fax: +81 92 642 4214; Email: yfujiscb{at}mbox.nc.kyushu-u.ac.jp
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