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Human Molecular Genetics, 2000, Vol. 9, No. 20 3055-3064
© 2000 Oxford University Press

Schwann cells harbor the somatic NF1 mutation in neurofibromas: evidence of two different Schwann cell subpopulations

Eduard Serra1,+, Thorsten Rosenbaum2,+, Ursula Winner2, Rosa Aledo3, Elisabet Ars1, Xavier Estivill1, Hans-Gerd Lenard2 and Conxi Lázaro1,§

1Medical and Molecular Genetics Center-IRO, Hospital Duran i Reynals, Autovia de Castelldefels km 2.7 08907 L’Hospitalet de Llobregat, Barcelona, Spain, 2Department of Neuropediatrics, Heinrich-Heine-University, Children’s Hospital, Moorenstrasse 5, 40225 Düsseldorf, Germany and 3Department of Biochemistry, School of Health Sciences, International University of Catalonia, Sant Cugat del Vallès, Barcelona, Spain

Neurofibromas are one of the most characteristic features of neurofibromatosis type 1 (NF1), an inherited autosomal-dominant neurogenetic disorder affecting 1 in 3500 individuals worldwide. These benign tumors mainly consist of Schwann cells (SCs) and fibroblasts. Recent evidence demonstrates that somatic mutations at the NF1 gene are found in neurofibromas, but it has not been demonstrated whether SCs, fibroblasts and/or both cell types bear a somatic loss of NF1. We recently established a cell culture system that allows selective expansion of human SCs from neurofibromas. We cultured pure populations of SCs and fibroblasts derived from 10 neurofibromas with characterized NF1 mutations and found that SCs but not fibroblasts harbored a somatic mutation at the NF1 locus in all studied tumors. Furthermore, by culturing neurofibroma-derived SCs under different in vitro conditions we were able to obtain two genetically distinct SC subpopulations: NF1–/– and NF1+/–. These data strongly support the idea that NF1 mutations in SCs, but not in fibroblasts, correlate to neurofibroma formation and demonstrate that only a portion of SCs in neurofibromas have mutations in both NF1 alleles.

+ These authors contributed equally to this work

§ To whom correspondence should be addressed. Tel: +34 93 260 7775; Fax: +34 93 260 7776; Email: clazaro@iro.es


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