Human Molecular Genetics Advance Access published online on October 7, 2003
Human Molecular Genetics, doi:10.1093/hmg/ddg335
© 2003 by Oxford University Press
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1 Unité de Recherche en Génétique Humaine et Moléculaire, Centre de recherche Hôpital Saint François d'Assise, Le CHUQ, Québec, (Qc) G1L 3L5 and Département de biologie médicale, Faculté de médecine, Université Laval, Québec, Canada; Department of Biochemistry, McGill University, Montréal, PQ, Canada
* To whom correspondence should be addressed. E-mail: edward.khandjian{at}crsfa.ulaval.ca.
Fragile X Mental Retardation protein (FMRP) is an RNA-binding protein that contains multiple domains with apparently differential affinity to mRNA and to the ribonucleotide homopolymer poly(G). Attempts have been made to map the RNA-binding sites along the protein sequence in view to determine which of the KH1, KH2 and RGG domains are required to recognize and bind to RNA. While these studies have greatly contributed to the delineation of domains that bind homopolymers or mRNA in vitro, little is known concerning their implications in FMRP function(s) in vivo. To address this question, we have prepared a series of FMRP versions, in which each known in vitro functional domain has been individually deleted, leaving the rest of the protein intact. Constructs with deletions in the protein-protein interaction and RNA-binding as well as in the phosphorylation domains were expressed in STEK-KO cells lacking FMRP and their recruitment into polyribosomal mRNPs and their intra-cellular localization were determined. Our results indicate that the KH RNA-binding domains and the Protein-Protein Interacting domain are essential for FMRP to associate with polyribosomal mRNPs, while the RGG box and the phosphorylated domains are dispensable.
Article
Fragile X Mental Retardation Protein determinants required for its association with polyribosomal mRNPs
2 Unité de Recherche en Génétique Humaine et Moléculaire, Centre de recherche Hôpital Saint François d'Assise, Le CHUQ, Québec, (Qc) G1L 3L5 and Département de biologie médicale, Faculté de médecine, Université Laval, Québec, Canada
3 Unité de Recherche en Génétique Humaine et Moléculaire, Centre de recherche Hôpital Saint François d'Assise, 10 rue de l'Espinay, Le CHUQ, Québec, (Qc) G1L 3L5, PQ and Département de biologie médicale, Faculté de médecine, Université Laval, Québec, Canada
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