Skip Navigation



Human Molecular Genetics Advance Access published online on January 28, 2004
Human Molecular Genetics, doi:10.1093/hmg/ddh068
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
13/6/601    most recent
ddh068v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Abeyta, M. J.
Right arrow Articles by Firpo, M. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abeyta, M. J.
Right arrow Articles by Firpo, M. T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

©2004 Oxford University Press

Article

Unique Gene Expression Signatures Of Independently-Derived Human Embryonic Stem Cell Lines

Michael J. Abeyta 1, Amander T. Clark 1, Ryan T. Rodriguez 2, Megan S. Bodnar 2, Renee A. Reijo Pera 1*, and Meri T. Firpo 2

1 Center for Reproductive Sciences; Department of Obstetrics, Gynecology and Reproductive Sciences, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0546, USA; Departments of Physiology and Urology; Programs in Human Genetics, Cancer Genetics, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0546, USA; Developmental and Stem Cell Biology; University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0546, USA
2 Center for Reproductive Sciences; Department of Obstetrics, Gynecology and Reproductive Sciences, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0546, USA; Developmental and Stem Cell Biology; University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0546, USA

* To whom correspondence should be addressed. E-mail: Reijo{at}itsa.ucsf.edu.


  Abstract

Human embryonic stem cells (hESCs) have the potential to differentiate to diverse cell types. This ability endows hESCs with promise for the development of novel therapeutics, as well as promise for the development of a rigorous genetic system to probe human gene function. However, in spite of the impending utility of hESCs for clinical and basic applications, little is known about their fundamental properties. Recent reports have documented transcriptional profiles of mouse embryonic stem cells (mESCs), adult stem cells, and a single hESC line, H9. To date, however, the transcriptional profiles of independently-derived hESC lines have not been compared. In order to examine the similarities and differences in multiple hESC lines, we compared gene expression profiles of the HSF-1, HSF-6 and H9 lines. We found that the majority of genes examined were expressed in all three cell lines. However, we also observed that each line possessed a unique expression signature; the expression of many genes was limited to just one or two hESC lines. We suggest that the observed differences in gene expression between independently derived hESC lines may reflect inherent differences in the initial culture of each line and/or the underlying genetics of the embryos from which the lines were derived.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
R. Pal, S. Totey, M. K. Mamidi, V. S. Bhat, and S. Totey
Propensity of Human Embryonic Stem Cell Lines During Early Stage of Lineage Specification Controls Their Terminal Differentiation into Mature Cell Types
Experimental Biology and Medicine, October 1, 2009; 234(10): 1230 - 1243.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
C. R. Nicholas, S. L. Chavez, V. L. Baker, and R. A. Reijo Pera
Instructing an Embryonic Stem Cell-Derived Oocyte Fate: Lessons from Endogenous Oogenesis
Endocr. Rev., May 1, 2009; 30(3): 264 - 283.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M.-Y. Son, J. Kim, H.-W. Han, S.-M. Woo, Y. S. Cho, Y.-K. Kang, and Y.-M. Han
Expression profiles of protein tyrosine kinase genes in human embryonic stem cells
Reproduction, October 1, 2008; 136(4): 423 - 432.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
D. Xie, J. Cai, N.-Y. Chia, H. H. Ng, and S. Zhong
Cross-species de novo identification of cis-regulatory modules with GibbsModule: Application to gene regulation in embryonic stem cells
Genome Res., August 1, 2008; 18(8): 1325 - 1335.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
G. W. Yeo, N. Coufal, S. Aigner, B. Winner, J. A. Scolnick, M. C.N. Marchetto, A. R. Muotri, C. Carson, and F. H. Gage
Multiple layers of molecular controls modulate self-renewal and neuronal lineage specification of embryonic stem cells
Hum. Mol. Genet., April 15, 2008; 17(R1): R67 - R75.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
R. D. Morin, M. D. O'Connor, M. Griffith, F. Kuchenbauer, A. Delaney, A.-L. Prabhu, Y. Zhao, H. McDonald, T. Zeng, M. Hirst, et al.
Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells
Genome Res., April 1, 2008; 18(4): 610 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A.L Green, D.N Wells, and B Oback
Cattle Cloned from Increasingly Differentiated Muscle Cells
Biol Reprod, September 1, 2007; 77(3): 395 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Wu, J. Xu, Z. P. Pang, W. Ge, K. J. Kim, B. Blanchi, C. Chen, T. C. Sudhof, and Y. E. Sun
Integrative genomic and functional analyses reveal neuronal subtype differentiation bias in human embryonic stem cell lines
PNAS, August 21, 2007; 104(34): 13821 - 13826.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. L. Garcia-Perez, M. C.N. Marchetto, A. R. Muotri, N. G. Coufal, F. H. Gage, K. S. O'Shea, and J. V. Moran
LINE-1 retrotransposition in human embryonic stem cells
Hum. Mol. Genet., July 1, 2007; 16(13): 1569 - 1577.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
C. Allegrucci, Y.-Z. Wu, A. Thurston, C. N. Denning, H. Priddle, C. L. Mummery, D. Ward-van Oostwaard, P. W. Andrews, M. Stojkovic, N. Smith, et al.
Restriction landmark genome scanning identifies culture-induced DNA methylation instability in the human embryonic stem cell epigenome
Hum. Mol. Genet., May 15, 2007; 16(10): 1253 - 1268.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Adachi, C. Soeta-Saneyoshi, H. Sagara, and Y. Iwakura
Crucial Role of Bysl in Mammalian Preimplantation Development as an Integral Factor for 40S Ribosome Biogenesis
Mol. Cell. Biol., March 15, 2007; 27(6): 2202 - 2214.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
C. Allegrucci and L.E. Young
Differences between human embryonic stem cell lines
Hum. Reprod. Update, March 1, 2007; 13(2): 103 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. A. Byrne, S. M. Mitalipov, L. Clepper, and D. P. Wolf
Transcriptional Profiling of Rhesus Monkey Embryonic Stem Cells
Biol Reprod, December 1, 2006; 75(6): 908 - 915.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
L. Armstrong, O. Hughes, S. Yung, L. Hyslop, R. Stewart, I. Wappler, H. Peters, T. Walter, P. Stojkovic, J. Evans, et al.
The role of PI3K/AKT, MAPK/ERK and NF{kappa}{beta} signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis
Hum. Mol. Genet., June 1, 2006; 15(11): 1894 - 1913.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Poon, F. Clermont, M. T. Firpo, and R. J. Akhurst
TGF{beta} inhibition of yolk-sac-like differentiation of human embryonic stem-cell-derived embryoid bodies illustrates differences between early mouse and human development
J. Cell Sci., February 15, 2006; 119(4): 759 - 768.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. L. Olsen, D. L. Stachura, and M. J. Weiss
Designer blood: creating hematopoietic lineages from embryonic stem cells
Blood, February 15, 2006; 107(4): 1265 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
S. P. Raikwar, T. Mueller, and N. Zavazava
Strategies for Developing Therapeutic Application of Human Embryonic Stem Cells
Physiology, February 1, 2006; 21(1): 19 - 28.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
B. W. Sun, A. C. Yang, Y. Feng, Y. J. Sun, Y. f. Zhu, Y. Zhang, H. Jiang, C. L. Li, F. R. Gao, Z. H. Zhang, et al.
Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies
Hum. Mol. Genet., January 1, 2006; 15(1): 65 - 75.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
L. G. Puente, D. J. Borris, J.-F. Carriere, J. F. Kelly, and L. A. Megeney
Identification of Candidate Regulators of Embryonic Stem Cell Differentiation by Comparative Phosphoprotein Affinity Profiling
Mol. Cell. Proteomics, January 1, 2006; 5(1): 57 - 67.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. L. Smith, R. E. Everts, X. C. Tian, F. Du, L.-Y. Sung, S. L. Rodriguez-Zas, B.-S. Jeong, J.-P. Renard, H. A. Lewin, and X. Yang
Global gene expression profiles reveal significant nuclear reprogramming by the blastocyst stage after cloning
PNAS, December 6, 2005; 102(49): 17582 - 17587.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
T. Enver, S. Soneji, C. Joshi, J. Brown, F. Iborra, T. Orntoft, T. Thykjaer, E. Maltby, K. Smith, R. A. Dawud, et al.
Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells
Hum. Mol. Genet., November 1, 2005; 14(21): 3129 - 3140.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. A. Cowan, J. Atienza, D. A. Melton, and K. Eggan
Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells
Science, August 26, 2005; 309(5739): 1369 - 1373.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Ezashi, P. Das, and R. M. Roberts
Low O2 tensions and the prevention of differentiation of hES cells
PNAS, March 29, 2005; 102(13): 4783 - 4788.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. R. Rao and S. L. Stice
Gene Expression Profiling of Embryonic Stem Cells Leads to Greater Understanding of Pluripotency and Early Developmental Events
Biol Reprod, December 1, 2004; 71(6): 1772 - 1778.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.