Skip Navigation



Human Molecular Genetics Advance Access published online on June 9, 2004
Human Molecular Genetics, doi:10.1093/hmg/ddh173
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
13/15/1563    most recent
ddh173v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cheng, H.
Right arrow Articles by Swaroop, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, H.
Right arrow Articles by Swaroop, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Article

Photoreceptor specific nuclear receptor NR2E3 functions as a transcriptional activator in rod photoreceptors

Hong Cheng 1, Hemant Khanna 2, Edwin C. T. Oh 1, David Hicks 3, Kenneth P. Mitton 4, Anand Swaroop 5*

1 Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI; Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI
2 Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI
3 Laboratoire de Physiopathologie Cellulaire et Moléculaire de la Rétine, INSERM Université Louis Pasteur E9918 Centre Hospitalier Universitaire Régional, 67091 Strasbourg, France
4 Eye Research Institute, Oakland University, Rochester, MI
5 Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI; Department of Ophthalmology and Visual Sciences, W. K. Kellogg Eye Center, University of Michigan, 1000 Wall Street, Ann Arbor, MI 48105, USA; Department of Human Genetics, University of Michigan, Ann Arbor, MI

* To whom correspondence should be addressed. E-mail: swaroop{at}umich.edu.


  Abstract

NR2E3, a photoreceptor-specific orphan nuclear receptor, is believed to play a pivotal role in the differentiation of photoreceptors. Mutations in the human NR2E3 gene and its mouse ortholog are associated with enhanced S-cones and retinal degeneration. In order to gain insights into NR2E3 function, we performed temporal and spatial expression analysis, yeast two-hybrid screening, promoter activity assays and co-immunoprecipitation studies. Nr2e3 expression was preferentially localized to the rod, and not cone, photoreceptor nuclei in rodent retina. Yeast two-hybrid screening of a retinal cDNA library, using NR2E3 as the bait, identified another orphan nuclear receptor NR1D1 (Rev-erb{alpha}). The interaction of NR2E3 with NR1D1 was confirmed by GST-pulldown and co-immunoprecipitation experiments. In transient transfection studies using HEK 293 cells, both NR2E3 and NR1D1 activated the promoters of rod phototransduction genes synergistically with neural retina leucine zipper (NRL) and cone-rod homeobox (CRX). All four proteins, NR2E3, NR1D1, NRL and CRX, could be co-immunoprecipitated from the bovine retinal nuclear extract, suggesting their existence in a multi-protein transcriptional regulatory complex in vivo. Our results demonstrate that NR2E3 is involved in regulating the expression of rod photoreceptor-specific genes and support its proposed role in transcriptional regulatory network(s) during rod differentiation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
A. N. Riesenberg, Z. Liu, R. Kopan, and N. L. Brown
Rbpj Cell Autonomous Regulation of Retinal Ganglion Cell and Cone Photoreceptor Fates in the Mouse Retina
J. Neurosci., October 14, 2009; 29(41): 12865 - 12877.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
S. I. Pachydaki, C. C. Klaver, I. A. Barbazetto, M. S. Roy, P. Gouras, R. Allikmets, and L. A. Yannuzzi
Phenotypic Features of Patients With NR2E3 Mutations
Arch Ophthalmol, January 1, 2009; 127(1): 71 - 75.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Langmann, C. C. L. Lai, K. Weigelt, B. M. Tam, R. Warneke-Wittstock, O. L. Moritz, and B. H. F. Weber
CRX controls retinal expression of the X-linked juvenile retinoschisis (RS1) gene
Nucleic Acids Res., November 1, 2008; 36(20): 6523 - 6534.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. A. Smyth, D. Di Lorenzo, and B. N. Kennedy
A Novel, Evolutionarily Conserved Enhancer of Cone Photoreceptor-specific Expression
J. Biol. Chem., April 18, 2008; 283(16): 10881 - 10891.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
G.-H. Peng and S. Chen
Crx activates opsin transcription by recruiting HAT-containing co-activators and promoting histone acetylation
Hum. Mol. Genet., October 15, 2007; 16(20): 2433 - 2452.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. C. T. Oh, N. Khan, E. Novelli, H. Khanna, E. Strettoi, and A. Swaroop
From the Cover: Transformation of cone precursors to functional rod photoreceptors by bZIP transcription factor NRL
PNAS, January 30, 2007; 104(5): 1679 - 1684.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Khanna, M. Akimoto, S. Siffroi-Fernandez, J. S. Friedman, D. Hicks, and A. Swaroop
Retinoic Acid Regulates the Expression of Photoreceptor Transcription Factor NRL
J. Biol. Chem., September 15, 2006; 281(37): 27327 - 27334.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
H. Cheng, T. S. Aleman, A. V. Cideciyan, R. Khanna, S. G. Jacobson, and A. Swaroop
In vivo function of the orphan nuclear receptor NR2E3 in establishing photoreceptor identity during mammalian retinal development
Hum. Mol. Genet., September 1, 2006; 15(17): 2588 - 2602.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Srinivas, L. Ng, H. Liu, L. Jia, and D. Forrest
Activation of the Blue Opsin Gene in Cone Photoreceptor Development by Retinoid-Related Orphan Receptor {beta}
Mol. Endocrinol., August 1, 2006; 20(8): 1728 - 1741.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Garelli, N. P. Rotstein, and L. E. Politi
Docosahexaenoic Acid Promotes Photoreceptor Differentiation without Altering Crx Expression.
Invest. Ophthalmol. Vis. Sci., July 1, 2006; 47(7): 3017 - 3027.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
B. Chang, H. Khanna, N. Hawes, D. Jimeno, S. He, C. Lillo, S. K. Parapuram, H. Cheng, A. Scott, R. E. Hurd, et al.
In-frame deletion in a novel centrosomal/ciliary protein CEP290/NPHP6 perturbs its interaction with RPGR and results in early-onset retinal degeneration in the rd16 mouse
Hum. Mol. Genet., June 1, 2006; 15(11): 1847 - 1857.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
O. Yaron, C. Farhy, T. Marquardt, M. Applebury, and R. Ashery-Padan
Notch1 functions to suppress cone-photoreceptor fate specification in the developing mouse retina
Development, April 1, 2006; 133(7): 1367 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Akimoto, H. Cheng, D. Zhu, J. A. Brzezinski, R. Khanna, E. Filippova, E. C. T. Oh, Y. Jing, J.-L. Linares, M. Brooks, et al.
From the Cover: Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors
PNAS, March 7, 2006; 103(10): 3890 - 3895.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. R. Roberts, A. Hendrickson, C. R. McGuire, and T. A. Reh
Retinoid X Receptor {gamma} Is Necessary to Establish the S-opsin Gradient in Cone Photoreceptors of the Developing Mouse Retina
Invest. Ophthalmol. Vis. Sci., August 1, 2005; 46(8): 2897 - 2904.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. R. Graham, P. A. Overbeek, and J. D. Ash
Leukemia Inhibitory Factor Blocks Expression of Crx and Nrl Transcription Factors to Inhibit Photoreceptor Differentiation
Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2601 - 2610.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Qian, N. Esumi, Y. Chen, Q. Wang, I. Chowers, and D. J. Zack
Identification of regulatory targets of tissue-specific transcription factors: application to retina-specific gene regulation
Nucleic Acids Res., June 20, 2005; 33(11): 3479 - 3491.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
X. Shu, A.M. Fry, B. Tulloch, F.D.C. Manson, J.W. Crabb, H. Khanna, A.J. Faragher, A. Lennon, S. He, P. Trojan, et al.
RPGR ORF15 isoform co-localizes with RPGRIP1 at centrioles and basal bodies and interacts with nucleophosmin
Hum. Mol. Genet., May 1, 2005; 14(9): 1183 - 1197.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
G.-H. Peng, O. Ahmad, F. Ahmad, J. Liu, and S. Chen
The photoreceptor-specific nuclear receptor Nr2e3 interacts with Crx and exerts opposing effects on the transcription of rod versus cone genes
Hum. Mol. Genet., March 15, 2005; 14(6): 747 - 764.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Chen, A. Rattner, and J. Nathans
The Rod Photoreceptor-Specific Nuclear Receptor Nr2e3 Represses Transcription of Multiple Cone-Specific Genes
J. Neurosci., January 5, 2005; 25(1): 118 - 129.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. M. Nishiguchi, J. S. Friedman, M. A. Sandberg, A. Swaroop, E. L. Berson, and T. P. Dryja
Recessive NRL mutations in patients with clumped pigmentary retinal degeneration and relative preservation of blue cone function
PNAS, December 21, 2004; 101(51): 17819 - 17824.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Friedman, H. Khanna, P. K. Swain, R. DeNicola, H. Cheng, K. P. Mitton, C. H. Weber, D. Hicks, and A. Swaroop
The Minimal Transactivation Domain of the Basic Motif-Leucine Zipper Transcription Factor NRL Interacts with TATA-binding Protein
J. Biol. Chem., November 5, 2004; 279(45): 47233 - 47241.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. W. Pickrell, X. Zhu, X. Wang, and C. M. Craft
Deciphering the Contribution of Known cis-Elements in the Mouse Cone Arrestin Gene to its Cone-Specific Expression
Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 3877 - 3884.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Yu, S. He, J. S. Friedman, M. Akimoto, D. Ghosh, A. J. Mears, D. Hicks, and A. Swaroop
Altered Expression of Genes of the Bmp/Smad and Wnt/Calcium Signaling Pathways in the Cone-only Nrl-/- Mouse Retina, Revealed by Gene Profiling Using Custom cDNA Microarrays
J. Biol. Chem., October 1, 2004; 279(40): 42211 - 42220.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S. G. Jacobson, A. Sumaroka, T. S. Aleman, A. V. Cideciyan, S. B. Schwartz, A. J. Roman, R. R. McInnes, V. C. Sheffield, E. M. Stone, A. Swaroop, et al.
Nuclear receptor NR2E3 gene mutations distort human retinal laminar architecture and cause an unusual degeneration
Hum. Mol. Genet., September 1, 2004; 13(17): 1893 - 1902.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.