Skip Navigation



Human Molecular Genetics Advance Access published online on November 8, 2005
Human Molecular Genetics, doi:10.1093/hmg/ddi414
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
14/24/3899    most recent
ddi414v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Reynolds, N.
Right arrow Articles by Cooke, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reynolds, N.
Right arrow Articles by Cooke, H. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved
Received September 23, 2005
Revised November 2, 2005
Accepted November 2, 2005

Article

Dazl binds in vivo to specific transcripts and can regulate the pre-meiotic translation of Mvh in germ cells

Nicola Reynolds 1, Brian Collier 2, Klio Maratou 1, Victoria Bingham 1, Robert M. Speed 1, Mary Taggart 1, Colin A. Semple 1, Nicola K. Gray 2, and Howard J. Cooke 1*

1 MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, Scotland, EH4 2XU, UK
2 MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, Scotland, EH4 2XU, UK; School of Molecular and Clinical Medicine, 3rd Floor Outpatients Department, Western General Hospital, University of Edinburgh, Edinburgh, Scotland, EH4 2XU, UK

* To whom correspondence should be addressed.
Howard J. Cooke, E-mail: Howard.Cooke{at}hgu.mrc.ac.uk


  Abstract

Gametogenesis is a complex process subject to strict controls at both the levels of transcription and translation. Members of a family of conserved RNA binding proteins encoded by the DAZ genes are required for the translational regulation of gene expression essential for this process. Although loss of DAZ family genes is associated with infertility in several organisms including humans, the identity of the transcripts regulated in vivo is unknown. Using a combination of immunoprecipitation and microarray analysis, we have identified a number of mRNAs that are bound by the murine Dazl protein both in vivo and in vitro. Sequence analysis shows that these transcripts contain binding sites for Dazl, which have been conserved during evolution between human, rat and mouse. We have focussed on Mvh, a gene which is essential for male gametogenesis and show that Dazl stimulates translation via the Mvh 3' UTR. Finally, we show that germ cells of Dazl null mice contain reduced levels of Mvh protein, indicating that Dazl-mediated regulation of Mvh translation is crucial for mammalian spermatogenesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
N. Reynolds, B. Collier, V. Bingham, N. K. Gray, and H. J. Cooke
Translation of the synaptonemal complex component Sycp3 is enhanced in vivo by the germ cell specific regulator Dazl
RNA, July 1, 2007; 13(7): 974 - 981.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
Y. S. Cheng, P. L. Kuo, Y. N. Teng, T. Y. Kuo, C. L. Chung, Y. H. Lin, R. W. Liao, J. S. N. Lin, and Y. M. Lin
Association of spermatogenic failure with decreased CDC25A expression in infertile men
Hum. Reprod., September 1, 2006; 21(9): 2346 - 2352.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
Y.-T. Lin and P. H. Yen
A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein
RNA, August 1, 2006; 12(8): 1486 - 1493.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
N. A Smirnova, P. J Romanienko, P. P Khil, and R D. Camerini-Otero
Gene expression profiles of Spo11-/- mouse testes with spermatocytes arrested in meiotic prophase I
Reproduction, July 1, 2006; 132(1): 67 - 77.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.