Skip Navigation



Human Molecular Genetics Advance Access published online on March 6, 2006
Human Molecular Genetics, doi:10.1093/hmg/ddl047
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
15/8/1291    most recent
ddl047v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Aartsen, W. M.
Right arrow Articles by Wijnholds, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aartsen, W. M.
Right arrow Articles by Wijnholds, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved
Received November 1, 2005
Revised February 24, 2006
Accepted March 2, 2006

Article

Mpp4 recruits Psd95 and Veli3 towards the photoreceptor synapse

Wendy M. Aartsen 1, Albena Kantardzhieva 1, Jan Klooster 1, Agnes G.S.H. van Rossum 1, Serge A. van de Pavert 1, Inge Versteeg 1, Bob Nunes Cardozo 1, Felix Tonagel 2, Susanne C. Beck 2, Naoyuki Tanimoto 2, Mathias W. Seeliger 2, and Jan Wijnholds 3 *

1 The Netherlands Institute for Neurosciences, Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands
2 Retinal Electrodiagnostics Research Group, Department of Ophthalmology, University of Tübingen, Schleichstr. 12-16, 72076 Tübingen, Germany
3 Department of Neuromedical Genetics, The Netherlands Institute for Neurosciences, Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands

* To whom correspondence should be addressed.
Jan Wijnholds, E-mail: j.wijnholds{at}nin.knaw.nl


  Abstract

Membrane-associated guanylate kinase (MAGUK) proteins function as scaffold proteins contributing to cell polarity and organizing signal transducers at the neuronal synapse membrane. The MAGUK protein Mpp4 is located in the retinal outer plexiform layer (OPL) at the presynaptic plasma membrane and presynaptic vesicles of photoreceptors. Additionally, it is located at the outer limiting membrane (OLM) where it might be involved in OLM integrity. In the Mpp4 knockout mice, loss of Mpp4 function only sporadically causes photoreceptor displacement, without changing the Crumbs (Crb) protein complex at the OLM, adherens junctions or synapse structure. Scanning laser ophthalmology revealed no retinal degeneration. The minor morphological effects suggest that Mpp4 is a candidate gene for mild retinopathies only. At the OPL, Mpp4 is essential for correct localization of Psd95 and Veli3 at the presynaptic photoreceptor membrane. Psd95 labeling is absent of presynaptic membranes in both rods and cones but still present in cone basal contacts and dendritic contacts. Total retinal Psd95 protein levels are significantly reduced which suggests Mpp4 to be involved in Psd95 turnover, whereas Veli3 proteins levels are not changed. These protein changes in the photoreceptor synapse did not result in an altered electroretinograph. These findings suggest that Mpp4 coordinates Psd95/Veli3 assembly and maintenance at synaptic membranes. Mpp4 is a critical recruitment factor to organize scaffolds at the photoreceptor synapse and is likely to be associated with synaptic plasticity and protein complex transport.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
N. A. Bulgakova and E. Knust
The Crumbs complex: from epithelial-cell polarity to retinal degeneration
J. Cell Sci., August 1, 2009; 122(15): 2587 - 2596.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Stohr, J. B. Heisig, P. M. Benz, S. Schoberl, V. M. Milenkovic, O. Strauss, W. M. Aartsen, J. Wijnholds, B. H. F. Weber, and H. L. Schulz
TMEM16B, A Novel Protein with Calcium-Dependent Chloride Channel Activity, Associates with a Presynaptic Protein Complex in Photoreceptor Terminals
J. Neurosci., May 27, 2009; 29(21): 6809 - 6818.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. Berger, N. A. Bulgakova, F. Grawe, K. Johnson, and E. Knust
Unraveling the Genetic Complexity of Drosophila stardust During Photoreceptor Morphogenesis and Prevention of Light-Induced Degeneration
Genetics, August 1, 2007; 176(4): 2189 - 2200.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Yang, B. Pawlyk, X.-H. Wen, M. Adamian, M. Soloviev, N. Michaud, Y. Zhao, M. A. Sandberg, C. L. Makino, and T. Li
Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals
Hum. Mol. Genet., May 1, 2007; 16(9): 1017 - 1029.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. A. van de Pavert, J. Meuleman, A. Malysheva, W. M. Aartsen, I. Versteeg, F. Tonagel, W. Kamphuis, C. J. McCabe, M. W. Seeliger, and J. Wijnholds
A Single Amino Acid Substitution (Cys249Trp) in Crb1 Causes Retinal Degeneration and Deregulates Expression of Pituitary Tumor Transforming Gene Pttg1
J. Neurosci., January 17, 2007; 27(3): 564 - 573.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Richard, R. Roepman, W. M. Aartsen, A. G.S.H. van Rossum, A. I. den Hollander, E. Knust, J. Wijnholds, and F. P.M. Cremers
Towards understanding CRUMBS function in retinal dystrophies
Hum. Mol. Genet., October 15, 2006; 15(suppl_2): R235 - R243.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. G.S.H. van Rossum, W. M. Aartsen, J. Meuleman, J. Klooster, A. Malysheva, I. Versteeg, J.-P. Arsanto, A. Le Bivic, and J. Wijnholds
Pals1/Mpp5 is required for correct localization of Crb1 at the subapical region in polarized Muller glia cells
Hum. Mol. Genet., September 15, 2006; 15(18): 2659 - 2672.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.