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Human Molecular Genetics Advance Access published online on April 13, 2006
Human Molecular Genetics, doi:10.1093/hmg/ddl098
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Published by Oxford University Press 2006
Received February 27, 2006
Revised April 3, 2006
Accepted April 3, 2006

Article

Mitochondrial localization of telomerase as a determinant for hydrogen peroxide-induced mitochondrial DNA damage and apoptosis

Janine Hertzog Santos 1 *, Joel N. Meyer 2, and Bennett Van Houten 2

1 Laboratory of Molecular Genetics, National Institute of Environmental and Health Sciences (NIEHS), National Institutes of Health (NIH). 111 Alexander Drive, MD D3-01, Research Triangle Park, NC, 27709; Currently at the Department of Pharmacology and Physiology, New Jersey Medical School of UMDNJ, Medical Sciences Building, H653, 185 South Orange Avenue, Newark, NJ, 07103, USA
2 Laboratory of Molecular Genetics, National Institute of Environmental and Health Sciences (NIEHS), National Institutes of Health (NIH). 111 Alexander Drive, MD D3-01, Research Triangle Park, NC, 27709

* To whom correspondence should be addressed.
Janine Hertzog Santos, E-mail: santosja{at}umdnj.edu


  Abstract

We have previously shown that the protein subunit of telomerase, hTERT, has a bona fide N-terminal mitochondrial targeting sequence, and that ectopic hTERT expression in human cells correlated with increase mtDNA after hydrogen peroxide treatment. In this study we show, using a loxP hTERT construct, that this increase in mtDNA damage following hydrogen peroxide exposure is dependent upon the presence of hTERT itself. Further experiments using a dominant negative hTERT mutant show that telomerase must be catalytically active to mediate the increase in mtDNA damage. Etoposide, but not methylmethanesulfate, also promotes mtDNA lesions in cells expressing active hTERT, indicating genotoxic specificity in this response. Fibroblasts expressing hTERT not only show a ~2-fold increase in mtDNA damage after oxidative stress but also suffer a 10-30-fold increase in apoptotic cell death as assayed by Annexin V staining, caspase 3 activation, and PARP cleavage. Mutations to the N-terminal mitochondrial leader sequence cause a complete loss of mitochondrial targeting without affecting catalytic activity. Cells carrying this mutated hTERT not only have significantly reduced levels of mtDNA damage following hydrogen peroxide treatment, but strikingly also do not shown any loss of viability or cell growth. Thus, localization of hTERT to the mitochondria renders cells more susceptible to oxidative stress-induced mtDNA damage and subsequent cell death, whereas nuclear-targeted hTERT, in the absence of mitochondrial localization, is associated with diminished mtDNA damage, increased cell survival and protection against cellular senescence.


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