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Human Molecular Genetics Advance Access published online on October 26, 2006
Human Molecular Genetics, doi:10.1093/hmg/ddl421
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© The Author 2006. Published by Oxford University Press. All rights reserved
Received July 19, 2006
Revised October 20, 2006
Accepted October 20, 2006

Article

The epilepsy gene LGI1 encodes a secreted glycoprotein that binds to the cell surface

Ma Salomé Sirerol-Piquer 1, Ana Ayerdi-Izquierdo 1, José Manuel Morante-Redolat 1, Vicente Herranz-Pérez 1, Kristy Favell 2, Carlo Nobile 3, Philip A. Barker 2, and Jordi Pérez-Tur 1 *

1 Unitat de Genètica Molecular. Departament de Genòmica i Proteòmica. Institut de Biomedicina de València-CSIC, C/ Jaume Roig, 11 E46010 València (Spain)
2 Montreal Neurological Institute, McGill University, Montreal, Quebec (Canada)
3 CNR-Istituto di Neuroscienze, Sezione di Padova, Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Padua, (Italy)

* To whom correspondence should be addressed.
Jordi Pérez-Tur, E-mail: jpereztur{at}ibv.csic.es


  Abstract

Autosomal dominant lateral temporal epilepsy (ADTLE) is a partial epilepsy caused by mutations in LGI1, a multidomain protein of unknown function. To begin to understand the biological function of LGI1, we have determined its pattern of glycosylation, subcellular expression and capacity for secretion. LGI1 is expressed as two different isoforms in the brain and we show that the long isoform is a secreted protein whereas the short isoform is retained in an intracellular pool. ADLTE-related mutants of the long form are defective for secretion and are retained in the endoplasmic reticulum and Golgi complex. Finally, we show that normal secreted LGI1 specifically binds to the cell surface of differentiated PC12 cells. We propose that LGI1 is a secreted factor important for neuronal development and that ADTLE is a disease that results from the loss of regulation in the protein available either extracellular or intracellularly.


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[Abstract] [Full Text] [PDF]


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