Skip Navigation



Human Molecular Genetics Advance Access published online on August 4, 2008
Human Molecular Genetics, doi:10.1093/hmg/ddn222
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
17/21/3263    most recent
ddn222v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Parker-Katiraee, L.
Right arrow Articles by Scherer, S. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parker-Katiraee, L.
Right arrow Articles by Scherer, S. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Dynamic variation in allele-specific gene expression of Paraoxonase-1 in murine and human tissues

Layla Parker-Katiraee1,2, Eleni Bousiaki3, David Monk3, Gudrun E. Moore3, Kazuhiko Nakabayashi1,4 and Stephen W. Scherer1,2,*

1 Department of Genetics and Genomic Biology, Hospital for Sick Children, Toronto M5G 1X8, Canada 2 Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada 3 Institute of Child Health, 30 Guilford Street, London, WC1 N 1EH, UK

* To whom correspondence should be addressed Corresponding author: Stephen W. Scherer The Hospital for Sick Children MaRS Centre - East Tower 101 College Street, Room 14-706 Toronto, Ontario, M5G 1L7, Canada Telephone: (416) 813-7613 Fax: (416) 813-8319 Email: steve{at}genet.sickkids.on.ca

Received April 29, 2008; Revised July 11, 2008; Accepted July 30, 2008

Differential allelic expression has been shown to be common in mice, humans, and maize, and variability in the expression of polymorphic alleles has been associated with human disease. Here, we describe the differential expression pattern of Paraoxonase-1, a gene involved in lipid metabolism and implicated in the formation of atherosclerotic lesions. We measured the expression of the murine Paraoxonase-1 gene (Pon1) in livers at different stages of embryonic development using F1 hybrid crosses and quantified the transcriptional level of both parental alleles. Using human fetal tissues, we analysed the expression of the human orthologue (PON1) and found monoallelic or preferential allelic expression in 6/7 and 4/4 samples from liver and pancreas, respectively. We observed that Pon1 does not show a parent-of-origin preference in its allelic expression, but has dramatic variations in allele specific expression occurring throughout development. This study has important repercussions in the analysis of haplotypes at disease loci, since it implies that the expression of polymorphic alleles can be unequal and dynamic.

4 Current Address: Department of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo, Japan


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.