Human Molecular Genetics Advance Access published online on January 15, 2009
Human Molecular Genetics, doi:10.1093/hmg/ddp023
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Tannic acid facilitates expression of the polypyrimidine tract binding protein and alleviates deleterious inclusion of CHRNA1 exon P3A due to an hnRNP H-disrupting mutation in congenital myasthenic syndrome
1 Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan 2 Department of Neurology and Neuromuscular Research Laboratory, Mayo Clinic, Rochester, Minnesota
* To whom correspondence should be addressed at: Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan, Tel: +81 527442446; Fax: +81 527442449; Email: ohnok{at}med.nagoya-u.ac.jp
Received October 10, 2008; Revised December 5, 2008; Accepted January 12, 2009
We recently reported that the intronic splice-site mutation IVS3-8G>A of CHRNA1 that encodes the muscle nicotinic acetylcholine receptor 
subunit disrupts binding of a splicing repressor, hnRNP H. This, in turn, results in exclusive inclusion of the downstream exon P3A. The P3A(+) transcript encodes a nonfunctional subunit that comprises 50% of the transcripts in normal human skeletal muscle, but its functional significance remains undetermined. In an effort to search for a potential therapy, we screened off-label effects of 960 bioactive chemical compounds and found that tannic acid ameliorates the aberrant splicing due to IVS3-8G>A but without altering the expression of hnRNP H. Therefore we searched for another splicing trans-factor. We found that the polypyrimidine tract binding protein (PTB) binds close to the 3’ end of CHRNA1 intron 3, that PTB induces skipping of exon P3A, and that tannic acid increases the expression of PTB in a dose-dependent manner. Deletion assays of the PTB promoter region revealed the tannic acid-responsive element is between positions -232 and -74 from the translation initiation site. These observations open the door to discovery of novel therapies based on PTB overexpression and to detecting possible untoward effects of the overexpression.