Human Molecular Genetics Advance Access published online on February 10, 2009
Human Molecular Genetics, doi:10.1093/hmg/ddp069
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A heteroplasmic, not homoplasmic, mitochondrial DNA mutation promotes tumorigenesis via alteration in reactive oxygen species generation and apoptosis
1 Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio, San Antonio, Texas 78229, USA 2 Wenzhou Medical College, Wenzhou 325035, China
* Corresponding author: Yidong Bai, PhD, Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio, San Antonio, Texas 78229 Telephone: (210)-567-0561 Fax: (210)-567-3803 E-mail: baiy{at}uthscsa.edu
Received December 18, 2008; Revised January 16, 2009; Accepted February 4, 2009
Mitochondrial alteration has been long proposed to play a major role in tumorigenesis. Recently, mitochondrial DNA (mtDNA) mutations have been found in a variety of cancer cells. In this study, we examined the contribution of mtDNA mutation and mitochondrial dysfunction in tumorigenesis first using human cell lines carrying a frame-shift at NADH dehydrogenase (respiratory complex I) subunit 5 gene (ND5); the same homoplasmic mutation was also identified in a human colorectal cancer cell line earlier. With increasing mutant ND5 mtDNA content, respiratory function including oxygen consumption and ATP generation through oxidative phosphorylation declined progressively, while lactate production and dependence on glucose increased. Interestingly, the reactive oxygen species (ROS) levels and apoptosis exhibited antagonistic pleiotropy associated with mitochondrial defects. Further more, the anchorage dependence phenotype and tumor-forming capacity of cells carrying wild-type and mutant mtDNA were tested by growth assay in soft agar and subcutaneous implantation of the cells in nude mice. Surprisingly, the cell line carrying the heteroplasmic ND5 mtDNA mutation showed significantly enhanced tumor growth, while cells with homoplasmic form of the same mutation inhibited tumor formation. Similar results were obtained from the analysis of a series of mouse cell lines carrying a nonsense mutation at ND5 gene. Our results indicate that the mtDNA mutations might play an important role in early stage of cancer development, possibly through alteration of ROS generation and apoptosis.
The authors wish it to be known that the first two authors should be considered the joint first authors.
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