Human Molecular Genetics Advance Access published online on April 9, 2009
Human Molecular Genetics, doi:10.1093/hmg/ddp180
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RNA Transcripts, miRNA-sized Fragments, and Proteins Produced from D4Z4 Units: New Candidates for the Pathophysiology of Facioscapulohumeral Dystrophy
1 Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA 2 Department of Medicine, University of Washington, Seattle, WA 98195, USA 3 Department of Human Genetics, Leiden Medical Center, Leiden, The Netherlands 4 Department of Biological Chemistry, University of California, Irvine, CA 92697 USA 5 Department of Neurology, University of Rochester, Rochester, NY 14642, USA 6 Department of Neurology, University of Washington, Seattle, 98195, USA
* Corresponding Author: Stephen J Tapscott Fred Hutchinson Cancer Research Center, Mailstop C3-168, 1100 Fairview Ave North, Seattle, WA 98109, Ph: 206-667-4499 FAX: 206-667-6524 Email: stapscot{at}fhcrc.org
Received January 5, 2009; Revised March 12, 2009; Accepted April 6, 2009
Deletion of a subset of the D4Z4 macrosatellite repeats in the subtelomeric region of chromosome 4q causes facioscapulohumeral muscular dystrophy (FSHD) when occurring on a specific haplotype of 4qter (4qA161). Several genes have been examined as candidates for causing FSHD, including the DUX4 homeobox gene in the D4Z4 repeat, but none have been definitively shown to cause the disease, nor has the full extent of transcripts from the D4Z4 region been carefully characterized. Using strand-specific RT-PCR, we have identified several sense and antisense transcripts originating from the 4q D4Z4 units in wild-type and FSHD muscle cells. Consistent with prior reports, we find that the DUX4 transcript from the last (most telomeric) D4Z4 unit is polyadenylated and has two introns in its 3-prime untranslated region. In addition, we show that this transcript generates (i) small si/miRNA-sized fragments, (ii) uncapped, polyadenylated 3-prime fragments that encode the conserved C-terminal portion of DUX4, and (iii) capped and polyadenylated mRNAs that contain the double-homeobox domain of DUX4 but splice-out the C-terminal portion. Transfection studies demonstrate that translation initiation at an internal methionine can produce the C-terminal polypeptide and developmental studies show that this peptide inhibits myogenesis at a step between MyoD transcription and the activation of MyoD target genes. Together, we have identified new sense and anti-sense RNA transcripts, novel mRNAs, and mi/siRNA-sized RNA fragments generated from the D4Z4 units that are new candidates for the pathophysiology of FSHD.
+ These authors contributed equally to the study
Genbank sequence accession number: FJ439133
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