Cover: The image shows a single myofiber isolated from fast-twitch muscle of a muscle-specific autophagy-deficient mouse. The suppression of autophagy in skeletal muscle is associated with the accumulation of autophagic substrates. One such substrate is polyubiquitin-binding protein p62, also known as sequestosome SQSTM1. This protein serves as an adaptor molecule mediating the degradation of polyubiquitinated proteins by the autophagic pathway, which terminates at lysosomes. The fiber was analyzed by confocal microscopy after double-staining for LAMP1 (Lysosomal-associated membrane protein 1; a lysosomal marker shown in red) and for P62/SQSTM1 (shown in green); nuclei are stained in blue on a merged image. See N. Raben et al., pp. 3897-3908.
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