Cover: Transient expression of the novel MID1 N-terminal mutant, E115X, in Cos1 cells as a GFP fusion protein shows that the E115X mutant protein does not colocalize with the microtubule network (red; 
-tubulin) as observed for the wild-type MID1. Instead, the protein (green / yellow) is found throughout the nucleus (blue; DAPI stain) and cytoplasm. This disrupted microtubule association is distinct from the cytoplasmic clumping seen with all C-terminal mutants previously identified in patients with X-linked Opitz syndrome and supports the conclusion that loss of function of MID1 is the underlying cause of the disorder. See Report by Cox et al. in this issue, pp. 2553-2562.
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